Multiplexed tat-Targeting CRISPR-Cas9 Protects T Cells from Acute HIV-1 Infection with Inhibition of Viral Escape

HIV-1 cure strategy by means of proviral knock-out using CRISPR-Cas9 has been hampered by the emergence of viral resistance against the targeting guide RNA (gRNA).Here, we proposed multiple, concentrated gRNA attacks against HIV-1 regulatory genes to block viral escape.The T cell line were EDC transduced with single and multiple gRNAs targeting HIV

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A Coarse-to-Fine DLG and TLS Data Registration Method

Terrestrial laser scanning (TLS) is Vinyl an efficient tool to capture the point cloud for facade modeling.However, it is not easy to obtain the position by Global Navigation Satellite System (GNSS) as occluded by high buildings, which would lead to a problem in orienting the TLS data to a target coordinate system.In this article, we propose a coar

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